Using montevardi app for hyperspectral unmixing of 3d tif stacks
Hello, I am a first time user of this toolbox for the purpose of unmixing some fluorescence images.
I have 2 tif stacks each of dimension 51251232 where 32 is the number of frames. They are of different wavelengths. I'd like to know how to use this toolbox to unmix these stacks.
I tried to make a hyperstack using ImageJ with the 2 channel image stacks above. Choosing 512 mb as RAM, i click 'execute' on the EndmemberNumberEstimation page of montevardi application. The end members estimates comes up as 1 with "virtual dimensionality" algorithm. Next, i select the VertexComponentAnalysis" and run it with the hyperstack. This gives the error: : Invalid Matrix size. Number of columns must be the same as the image number of channels.
Can someone please tell me how to use this app with the above image stacks ?